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Fig. 6 | Phytopathology Research

Fig. 6

From: The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearum

Fig. 6

FgBzip16 targets fatty acid biosynthesis and metabolism-related genes. a EMSA assays for the binding of FgBzip16 protein with putative promoter sequences of FGSG_05321 and FGSG_03244, respectively. The 1500 bp DNA fragment of the putative promoter sequence of each gene was incubated in the absence or presence of purified FgBzip16 protein. GST protein or proteinase K was added after the incubation of FgBzip16 protein with DNA fragment as control. DNA–protein complexes were separated by 1.5% agarose gel electrophoresis and photographed. b EMSA assays with a gradient concentration of FgBzip16 protein

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