The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearum

Table 2 Expression changes of the genes involved in fatty acid biosynthesis and metabolism in F. graminearum FgBZIP16 deletion mutant by quantitative real-time PCR

Pathway	Accession number	Putative function	Fold change in gene expression^a
Fatty acid biosynthesis	FGSG_05322	fatty acid synthase subunit beta dehydratase	0.692*
	FGSG_05321	fatty acid synthase subunit alpha reductase	0.191*
	FGSG_07226	3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial precursor	1.483*
	FGSG_02324	hypothetical protein similar to type I polyketide synthase	14.914*
	FGSG_02210	conserved hypothetical protein	0.431*
	FGSG_07223	hypothetical protein similar to short chain dehydrogenase family protein	1.016
	FGSG_03838	conserved hypothetical protein	0.711*
	FGSG_01857	hypothetical protein similar to 3-oxoacyl-acyl-carrier-protein reductase	1.547*
	FGSG_08816	conserved hypothetical protein	0.286*
	FGSG_11409	conserved hypothetical protein	0.034*
Fatty acid metabolism	FGSG_01419	hypothetical protein similar to AMP-binding protein	5.325*
	FGSG_13860	conserved hypothetical protein	3.638*
	FGSG_02287	hypothetical protein similar to acyl-CoA oxidase	10.254*
	FGSG_12573	hypothetical protein similar to enoyl-CoA hydratase/isomerase family protein	4.344*
	FGSG_05551	hypothetical protein similar to peroxisomal D3,D2-enoyl-CoA isomerase	1.442*
	FGSG_07659	hypothetical protein similar to fadD36	0.780*
	FGSG_03244	hypothetical protein similar to enoyl-CoA hydratase/isomerase family protein	15.687*
	FGSG_01581	conserved hypothetical protein	2.536*
	FGSG_03546	hypothetical protein similar to dehydrogenase	6.692*
	FGSG_13111	enoyl-CoA hydratase, mitochondrial precursor	1.634*

^aFold-change value represents the fold expression in FgBZIP16 deletion mutant ΔFgbzip16 as compared with that in the wild-type strain PH-1. The asterisks indicate a significant difference, at P < 0.01