Fig. 2From: The E2 ubiquitin-conjugating enzyme CfRad6 regulates the autophagy and pathogenicity of Colletotrichum fructicola on Camellia oleiferaCfRad6 interacts with CfRad18, CfBre1, and CfUbr1. The pGADT7 and pGBKT7 vectors, fused with the related genes, were co-transformed into the yeast strain AH109. Ten-fold serial dilutions of transformants were spotted in equal volume (5 μL) on synthetic two-deficient media (SD-Leu-Trp) and synthetic quadruple dropout selection media (SD-Leu-Trp-Ade-His). Leu: leucine; Trp: tryptophan: Ade, adenine; His: histidineBack to article page