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Fig. 4 | Phytopathology Research

Fig. 4

From: The E2 ubiquitin-conjugating enzyme CfRad6 regulates the autophagy and pathogenicity of Colletotrichum fructicola on Camellia oleifera

Fig. 4

CfRad6 negatively regulates autophagy. a Micrographs of GFP-CfAtg8 labeled autophagosomes in the WT and ΔCfrad6 mutant strains. b Statistical analysis of autophagosome numbers in the WT and ΔCfrad6 mutant strains. On average, more than 50 hyphal tips (about 50 μm in length) were observed. The assays were repeated three times, and the results were similar. c Immunoblot analysis of GFP-CfAtg8 proteolysis in the WT and ΔCfrad6 mutant. The upper lanes point to the full GFP-Atg8 (46 kDa), and the lower lanes point to the free GFP (26 kDa). The autophagy level was estimated by calculating the amount of free GFP relative to the total amount of full GFP-CfAtg8 plus free GFP. d Micrographs of GFP-CfAtg8 labeled autophagosomes in WT with or without MG132 treatment. e Statistical analysis of autophagosome numbers in WT with or without MG132 treatment. f Immunoblot analysis of GFP-CfAtg8 proteolysis in the WT with or without MG132 treatment

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Phytopathology Research

ISSN: 2524-4167