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Fig. 6 | Phytopathology Research

Fig. 6

From: Molecular characterization of a novel polerovirus from bitter gourd plants and dynamic subcellular localization of the virus-encoded proteins

Fig. 6

Dynamic protein accumulation of BYCV-encoded viral proteins. Agrobacterium cultures expressing P0-YFP, P1-YFP, P1-P2-YFP, P3a-YFP, P3-YFP, P4-YFP, or P3-P5-YFP adjusted to an optical density at 600 nm (OD600) = 0.7 was infiltrated in N. benthamiana leaves. Total proteins were extracted from P0-YFP, P1-YFP, P1-P2-YFP, P3a-YFP, P3-YFP, P4-YFP, or P3-P5-YFP infiltrated leaves at 24, 48 and 72 hpi. An anti-GFP was used to detect antibody accumulation of protein P0-YFP (a), P1-YFP (b), P1-P2-YFP (c), P3a-YFP (d), P3-YFP (e), P4-YFP (f), or P3-P5-YFP (g) at 48, 72, and 96 hpi. A specific anti-Actin antibody was used as a loading in western blotting assays. h The protein accumulation from western blotting images was digitized by Image J, and the data at 24 hpi was set to 1

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Phytopathology Research

ISSN: 2524-4167