Fig. 3
XrvB is involved in the expression regulation of HrpG and XopN. a Deletion of xrvB enhanced the protein levels of HrpG and XopN. The Xoc strains GX01/HrpG::3 × Flag, ΔxrvB/HrpG::3 × Flag, GX01/XopN::3 × Flag, and ΔxrvB/XopN::3 × Flag were cultured in XOM2 or NB medium to mid-exponential growth phase (OD600 of 0.6), and total proteins were extracted. The proteins HrpG and XopN were detected using anti-Flag-tag antibody. RNAP served as a loading control. Proteins were quantified by Image J software. Band intensity of the proteins was normalized to RNAP levels. The mean value of the proteins in the wild-type strain was set to 1. Data are presented as means ± SD. **P < 0.01, significant difference from the wild-type strain, using Student's t-test. b Overexpression of xrvB reduced the protein levels of HrpG and XopN. Xoc strains GX01/HrpG::3 × Flag/pBBad22K, GX01/HrpG::3 × Flag/pBxrvB, GX01/XopN::3 × Flag/pBBad22K, and GX01/XopN::3 × Flag/pBxrvB were cultured in XOM2 or NB to an OD600 of 0.6, and total proteins were extracted. Data presented are means ± SD (n = 3). The mean value of the proteins in GX01/pBBad22K strain was set to 1. **P < 0.01, significant difference from the wild-type strain, using Student's t-test. c Deletion of XrvB significantly increased the transcription level of hrpG in the rich medium NB. Values given are the means ± SD (n = 3 biological repeats). N and X below the bar graph represent NB and XOM2 medium, respectively