Fig. 4
XrvB directly binds to the hrpG promoter region. a Electrophoretic mobility shift assay showed XrvB protein interacted with the hrpG promoter region. The gel shift assay was carried out using the 6-carboxyfluorescein (FAM)-labeled DNA probe of the hrpG promoter and His-XrvB protein. The migrated DNA–protein complexes and free probe are indicated by arrows. b Chromatin immunoprecipitation (ChIP) assay showing that XrvB binds to the hrpG promoter region in vivo. The Xoc strain GX01/XrvB::3 × Flag was grown in NB and XOM2 media at an OD600 value of 0.3. Template DNA from a non-conjugated ChIP sample was used as the input control and DNA fragments containing rpfG promoter were amplified as a negative control. Lane 1 and 5 are GX01 total DNA (PCR efficacy control); lane 2 and 6 are the GX01/XrvB::3 × Flag total DNA (input control); lane 3 and 7 are the eluted DNA of GX01/XrvB::3 × Flag using anti-HA antibody (negative control); lane 4 and 8 are the eluted DNA of GX01/XrvB::3 × Flag using anti-Flag antibody; lane 9 is mock control