Fig. 7
XrvB is required for the full virulence of Xoc. a Virulence test of Xoc strains. Six-week-old susceptible rice plants (Oryza sativa L. ssp. Japonica cultivar Nipponbare) were inoculated with bacterial suspensions of the Xoc strains by the leaf-infiltrating method. Symptoms were recorded at 14 dpi and lesion lengths were scored. Values are the mean ± SD (n = 25). Significance was determined by ANOVA and Dunnett's post hoc test for comparison to the wild type. *P < 0.05; n.s., not significant. The experiment was repeated three times with similar results. b Growth test of Xoc strains in planta and in complex media NB. The Xoc wild-type strain GX01, the xrvB deletion mutant ΔxrvB, the complemented strain CΔxrvB were infiltrated into the leaves of rice. Bacterial colony-forming units (CFU) were counted after incubation for 3 days. Data are shown as the mean ± SD (n = 3 biological repeats). For the growth assay in media, the Xoc strains were inoculated into 100 mL NB medium with the same final density of 0.01 (OD600). Samples were taken in triplicate at intervals of 4 h, diluted, and plated on NA plate. Bacterial CFU were counted after incubation for 3 days. Data are shown as the mean ± SD (n = 3 biological repeats)