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Fig. 2 | Phytopathology Research

Fig. 2

From: Systemic screening of Fusarium oxysporum candidate effectors reveals FoSSP17 that suppresses plant immunity and contributes to virulence

Fig. 2

Functional validation of SP of FoSSP17 and subcellular localization of FoSSP17 and FoSSP17ΔSP. a Verifying the SP function of FoSSP17 by yeast secretion system.Yeast Saccharomyces cerevisiae YTK12 strain and strains bearing the empty pSUC2 vector and the pSUC2 vector with the SP of Mg87 protein from Magnaporthe oryzae were used as negative controls; the strain harboring the pSUC2 vector with the SP of Avr1b protein from Phytophthora sojae was used as the positive control. b Subcellular localization of FoSSP17 and FoSSP17ΔSP. Leaves of N. benthamiana transiently expressing FoSSP17:eGFP and FoSSP17ΔSP:eGFP fusion proteins were examined by confocal microscopy. GFP was used as a control. The accumulated FoSSP17:eGFP is indicated with red arrows. Bars = 50 μm. c Western blot analysis of protein from N. benthamiana leaves expressing GFP, FoSSP17:eGFP, and FoSSP17ΔSP:eGFP. The same results were obtained with at least three biological replicates

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Phytopathology Research

ISSN: 2524-4167