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Fig. 3 | Phytopathology Research

Fig. 3

From: Systemic screening of Fusarium oxysporum candidate effectors reveals FoSSP17 that suppresses plant immunity and contributes to virulence

Fig. 3

Transient expression of FoSSP17 and FoSSP17ΔSP in N. benthamiana. a FoSSP17 and FoSSP17ΔSP suppressed BAX-induced cell death in N. benthamiana. Agrobacterium strains carrying pGR107-FoSSP17-GFP, pGR107-FoSSP17ΔSP-GFP, and pGR107-GFP (as negative control) were infiltrated separately in leaves 24 h before infiltration of the Agrobacterium expressing BAX at the same location. Dashed circles indicate the agroinfiltrated regions. Photos were taken 5 d after BAX treatment. Ultraviolet (UV) light was used to indicate the HR response. b Electrolyte leakage assay of N. benthamiana leaves co-expressing BAX with indicated proteins. Samples were collected 5 d after BAX treatment. Data shown are means ± SD calculated from three independent experiments. Statistical analyses were performed with One-way analysis of variance (ANOVA) and Tukey’s multiple comparisons test. Different letters indicate significant differences (P < 0.05). c Western blot analysis of the protein expression of FoSSP17 and FoSSP17ΔSP with GFP tag. Similar results were obtained from at least three biological replicates

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Phytopathology Research

ISSN: 2524-4167