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Fig. 4 | Phytopathology Research

Fig. 4

From: Systemic screening of Fusarium oxysporum candidate effectors reveals FoSSP17 that suppresses plant immunity and contributes to virulence

Fig. 4

FoSSP17 suppressed flg22-triggered PTI in N. benthamiana. a, b Flg22-induced ROS accumulation was suppressed in N. benthamiana leaves expressing FoSSP17:GFP. Samples were collected 12 h after infiltration with flg22. Photographs were taken after DAB staining (Bars = 200 μm). The relative area of DAB-stained leaf tissues was analyzed with ImageJ software. c, d FoSSP17:GFP suppressed flg22-induced callose deposition in tobacco leaves. Samples were collected 12 h after flg22 treatment. Photographs were taken after aniline blue staining (Bars = 200 μm). The number of callose spots per 1 mm2 was analyzed with ImageJ software. e Relative transcript levels of 5 defense-related genes. Total RNA was extracted from samples collected 12 h after flg22 treatment. NbActin gene was used as the reference gene for normalization. Relative transcript levels of genes in leaves expressing GFP are arbitrarily set to 1. For b, d, and e, data shown are means ± SD calculated from three biological replicates. Statistical analyses were performed with Student’s t-test. *P < 0.05; **P < 0.01

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Phytopathology Research

ISSN: 2524-4167