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Fig. 1 | Phytopathology Research

Fig. 1

From: Rice yellow stunt virus p3 protein enters the nucleus of leafhopper cell and localizes to viroplasm through interaction with N protein via importin α3-mediated pathway

Fig. 1

Subcellular localization of RYSV nonstructural protein P3 in N. cincticeps VCMs. a Fluorescence of N. cincticeps cells expressing GFP fused to P3. N. cincticeps cells transfected with the NC-CytoF1-GFP vector served as a control. Scale bars: 20 μm. b Confocal immunofluorescence micrographs showing the cellular localization of RYSV P3 in N. cincticeps cells at different stages of RYSV infection. Infected VCMs at 18, 36, 48, and 72 hpi were immunolabeled with P3-specific IgGs directly conjugated to rhodamine. Scale bars: 20 μm. c Electron micrographs showing the subcellular localization of RYSV P3 in RYSV-infected VCMs. Samples were immunolabeled with P3-specific antibodies as primary antibodies and treated with goat-anti-rabbit IgG conjugated to 15-nm-diameter gold particles as secondary antibodies. RYSV-infected VCMs were labeled with N-specific antibody separately as a control. Scale bars: 100 nm

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Phytopathology Research

ISSN: 2524-4167