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Fig. 2 | Phytopathology Research

Fig. 2

From: Rice yellow stunt virus p3 protein enters the nucleus of leafhopper cell and localizes to viroplasm through interaction with N protein via importin α3-mediated pathway

Fig. 2

In vitro and in vivo interactions of RYSV P3 and N. a Interactions between N and P3 in the yeast two-hybrid system. Yeast cells co-transformed with pGBKT7–53 and pGADT7-T were used as a positive control. b GST pull-down assay of the interaction of RYSV P3 with RYSV N. Bait protein N was fused with GST-tagged protein. Prey protein P3 was fused with His-tagged protein. Purified GST incubated with prey protein or purified His incubated with bait protein were used as controls. c Confocal immunofluorescence micrographs showing the colocalization of N-His and P3-Strep fusion proteins in Sf9 cells. Yeast cells expressing only N-His or P3-Strep served as a positive control. The samples were immunolabeled with Strep-tagged IgGs directly conjugated to FITC or His-tagged IgGs directly conjugated to rhodamine. Scale bars: 20 μm. d Confocal immunofluorescence micrographs showing the colocalization of N and P3 at different times after RYSV inoculation in host N. cincticeps cells. The cells were immunolabeled with N-specific IgGs directly conjugated to FITC or P3 specific IgGs directly conjugated to rhodamine. Scale bars: 20 μm

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Phytopathology Research

ISSN: 2524-4167