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Fig. 3 | Phytopathology Research

Fig. 3

From: Rice yellow stunt virus p3 protein enters the nucleus of leafhopper cell and localizes to viroplasm through interaction with N protein via importin α3-mediated pathway

Fig. 3

Knockdown of N and P3 by dsRNA treatment inhibits RYSV infection of VCMs. a VCMs were transfected with dsP3 or dsGFP and inoculated with RYSV. At 72 h post RYSV inoculation, the cells were immunolabeled with N-specific IgGs directly conjugated to FITC. Scale bars: 25 μm. Quantitative analysis of RYSV-infected cultured cells labeled with N-F antibodies at 72 h post RYSV inoculation, as revealed by confocal microscopy (1200 cells/condition were counted, mean ± SD; **P < 0.01). b RT-qPCR assay showing relative transcript levels of the N, P, M, and P3 genes after different treatments. Data represent means ± SD and were analyzed using Student’s t-test; *P < 0.05; **P < 0.01. c Immunoblot analysis of viral proteins from RYSV-infected VCMs with dsRNA treatments. N-, P-, M-, and P3-specific antibodies were used to detect the accumulation of different viral proteins at 84 hpi. Actin was used as the control and was detected with actin-specific antibody

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Phytopathology Research

ISSN: 2524-4167