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Fig. 4 | Phytopathology Research

Fig. 4

From: Rice yellow stunt virus p3 protein enters the nucleus of leafhopper cell and localizes to viroplasm through interaction with N protein via importin α3-mediated pathway

Fig. 4

Knockdown of P3 by dsRNA treatment inhibits RYSV infection of leafhoppers. a Immunohistochemistry showing the variation in the amount of RYSV in midguts of nonviruliferous N. cincticeps at 6 days after injection with RYSV crude extracts and dsRNA of P3 and N. Nonviruliferous N. cincticeps were injected with dsGFP and RYSV crude extracts as a positive control. All samples were stained with RYSV-N-rhodamine and the actin dye phalloidin (blue). Scale bars: 100 μm. b RT-qPCR assay showing relative transcript levels of N, P, M, and P3 after different dsRNA treatments. Three biological repeats were performed. Data represent means ± SD and were analyzed using Student’s t-test; *P < 0.05; **P < 0.01. c The accumulation of viral proteins from N. cincticeps injected with RYSV crude extracts and dsP3 or dsN. N-, P-, M- and P3-specific antibodies were used to detect N, P, M, and P3 protein, respectively, at 84 hpi. Actin was used as the control and was detected with actin-specific antibody

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Phytopathology Research

ISSN: 2524-4167