Fig. 1
From: Biocontrol and molecular characterization of Bacillus velezensis D against tobacco bacterial wilt
Bacillus velezensis D exhibited the best antibacterial activity against R. solanacearum. a, b Inhibition zone of rhizosphere bacteria against R. solanacearum. Antagonistic activities of rhizobacteria strains against R. solanacearum were analyzed through oxford cup method. 5 mL R. solanacearum suspensions (1 × 108 CFU/mL) were mixed with 1 L TTC medium and poured in plates. Sterile oxford cups (0.6 cm) impregnated with 150 μL rhizosphere bacterial suspensions (1 × 108 CFU/mL) were placed on the surface of solid medium. Finally, the diameters of inhibition zones were measured after incubating at 30℃ for 48 h. Sterile LB medium were used as control. Phylogenetic tree based on the 16S rDNA (c) and gyrB (d) sequences of B. velezensis D with Mega 7.0 software using neighbor-joining method. Data are presented as the mean ± SD based on three biological replicates, P-values were determined by LSD post-hoc test. Different letters represent significant difference