Fig. 4
From: Biocontrol and molecular characterization of Bacillus velezensis D against tobacco bacterial wilt
Crude extracts of B. velezensis D exhibited the antibacterial activity and inhibited motility of R. solanacearum. a Antagonistic activities of crude extracts against R. solanacearum were analyzed through oxford cup method. 5 mL R. solanacearum suspensions (1 × 108 CFU/mL) were mixed with 1 L TTC medium and poured in plates. Sterile oxford cups (0.6 cm) impregnated with 150 μL crude extracts (150 μg/mL) were placed on the surface of solid medium. Finally, the diameters of inhibition zones were measured after incubating at 30℃ for 48 h. Sterile LB medium were used as control. b The motility of R. solanacearum was observed in semisolid medium supplemented with sterile water, referred as a control. c The expression levels of target genes relating to motility of R. solanacearum at 24 h. serC was used as reference genes for normalization. Data are presented as the mean ± SD based on three biological replicates; P-values were determined by LSD post-hoc test. Different letters represent significant difference