Fig. 3

Fa and Fg isolates showed resistance to MBC and TEC. a Mycelial growth of Fg Lz114, Fa Lz503, Fa Lz167, and Fg Lz179 on PDA plates with the treatments of MBC and TEC at the 7th day after inoculation (Scale bars = 1 cm). Fg PH-1 was used as a control. CK means no addition of fungicide. Three biological replicates for each experiment. b Multiple protein sequence alignment of β2-tubulin protein sequence. Black arrows indicate the mutation sites. c Multiple protein sequence alignment of CYP51-B protein sequence. Fa Lz45, a control with same protein sequence, could not grow in 36 μg/mL TEC. d Relative expression levels of CYP51-A and CYP51-B in Fg Lz179. Mycelia were collected at 7th day after incubation on PDA plates supplied with 36 μg/mL TEC. There were three biological replicates. Asterisk indicates significance at P < 0.05