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Fig. 1 | Phytopathology Research

Fig. 1

From: Development and application of a self-assembling split-fluorescent protein toolkit to monitor geminiviral movement and infection in plant

Fig. 1

Establishment of a split super-folder GFP (sfGFP) system in Nicotiana benthamiana epidermal cells. a Construct design and complementation scheme of the split sfGFP system. b Genomic organization of tomato golden mosaic virus (TGMV) with DNA A under GenBank accession no. K02029 and DNA B under GenBank accession no. JF694491, respectively. Fluorescence images of N. benthamiana epidermal cells expressing N- or C-terminal GFP11-tagged coat protein (CP) c or BV1 protein d together with GFP1–10, respectively, at 48 h post-infiltration (hpi). Histone 2B (H2B)-RFP (RFP) and fibrillarin 1 (FIB1)-CFP (CFP) were used as nuclear and nucleolar markers respectively. GFP, GFP fluorescence; RFP, RFP fluorescence; CFP, CFP fluorescence. Scale bars: 10 μm

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Phytopathology Research

ISSN: 2524-4167