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Fig. 2 | Phytopathology Research

Fig. 2

From: Development and application of a self-assembling split-fluorescent protein toolkit to monitor geminiviral movement and infection in plant

Fig. 2

Viral protein labeling with GFP11 in the genome of tomato golden mosaic virus (TGMV) and viral movement assay in Nicotiana benthamiana leaves. a Labeling method with GFP11 at the N-terminal of coat protein (CP) in the genomic DNA A of TGMV (TGMV A) and viral inoculation strategy with GFP11-tagged infectious clones. b Confocal microscopy examination of the N. benthamiana epidermal cells shown in Fig. 2a. c Labeling method with GFP11 at the C-terminal of CP in the DNA A of TGMV and viral inoculation strategy with GFP11-tagged infectious clones. d Confocal microscopy examination of the N. benthamiana epidermal cells shown in Fig. 2c. e Labeling method with GFP11 at the N-terminal of BV1-encoded protein in the genomic DNA B of TGMV (TGMV B) and viral inoculation strategy with GFP11-tagged infectious clones. f Confocal microscopy examination of the N. benthamiana epidermal cells shown in Fig. 2e. g Labeling method with GFP11 at the C-terminal of BV1 protein in the genome of TGMV B and viral inoculation strategy with GFP11-tagged infectious clones. h Confocal microscopy examination of the N. benthamiana epidermal cells shown in Fig. 2g. Histone 2B (H2B)-RFP (RFP) and fibrillarin 1 (FIB1)-CFP (CFP) were used as nuclear and nucleolar markers, respectively. GFP, GFP fluorescence; RFP, RFP fluorescence; CFP, CFP fluorescence. Scale bars: 10 μm

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Phytopathology Research

ISSN: 2524-4167