Skip to main content
Fig. 3 | Phytopathology Research

Fig. 3

From: Development and application of a self-assembling split-fluorescent protein toolkit to monitor geminiviral movement and infection in plant

Fig. 3

Effects of the insertion sites of GFP11 on the viral pathogenicity of tomato golden mosaic virus (TGMV). a Disease symptoms detected in Nicotiana benthamiana plants agroinfiltrated with infectious clones of wild-type (WT) TGMV A (TGMV A-WT) or N- or C-terminal GFP11-tagged coat protein (CP) in TGMV DNA A together with WT TGMV DNA B (TGMV B-WT) at 14 days post-inoculation (dpi). b Western blot (WB) analysis of the infected leaves of N. benthamiana plants shown in Fig. 3a. c Disease symptoms detected in N. benthamiana plants agroinfiltrated with infectious clones of TGMV A-WT and TGMV B-WT or N- or C-terminal GFP11-tagged BV1 in TGMV DNA B at 14 dpi. d WB analysis of the infected leaves of N. benthamiana plants shown in Fig. 3c. In Fig. 3a, b, mock represents N. benthamiana plants agroinfiltrated with the empty vector pBINPLUS. In Fig. 3c, d, the TGMV CP protein was examined using an antibody against TGMV CP (α-TGMV CP) (top). WB analysis using an antibody to actin (α-Actin) served as a loading control (bottom)

Back to article page

Phytopathology Research

ISSN: 2524-4167