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Fig. 4 | Phytopathology Research

Fig. 4

From: Development and application of a self-assembling split-fluorescent protein toolkit to monitor geminiviral movement and infection in plant

Fig. 4

Construction of the stable genetic sfGFP system in Nicotiana benthamiana. a Phenotype of wild-type (WT) and GFP1–10 transgenic N. benthamiana plants agroinfiltrated with infectious clones of tomato golden mosaic virus (TGMV) whose genomic DNA A tagged with GFP11 at the N-terminus of coat protein (CP) (TGMV A-GFP11-CP) together WT TGMV B (TGMV B-WT) at 14 days post-inoculation (dpi). N. benthamiana plants agroinfiltrated with the empty vector pBINPLUS served as the mock. b Western blot (WB) analysis of the infected leaves of N. benthamiana plants shown in Fig. 4a. TGMV GFP11-CP and GFP1–10 proteins were detected using antibodies against TGMV CP (α-TGMV CP) (top) and GFP (α-GFP) (middle), respectively. WB analysis using an antibody to actin (α-Actin) served as a loading control (bottom). c and d Subcellular localization of TGMV in epidermal cells of leaves and leaf veins of WT and GFP1–10 transgenic N. benthamiana plants at 7 dpi. WT N. benthamiana plants agroinfiltrated with the TGMV A-GFP11-CP and TGMV B-WT served as the control. GFP, GFP fluorescence. Scale bars: 30 μm

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Phytopathology Research

ISSN: 2524-4167