Fig. 4
Pathogenicity assays on soybean leaves and seedlings. The parental wild-type isolate (WT) is P6497; EV is the control isolate of P. sojae transformed with an empty vector; LC is the PsLARP4_5 complemented isolate; KT1, KT18, and KT40 are PsLARP4_5 deletion transformants. a Soybean seedlings were inoculated with 10 µL of zoospore suspension (1 × 104 zoospores/mL) for 3 days. b Soybean seedlings were inoculated with a 5-mm mycelial plug for 3 days. c The P. sojae biomass in soybean seedlings inoculated with 10 µL of zoospore suspension (1 × 104 zoospores/mL). d The P. sojae biomass in soybean seedlings inoculated with a 5 mm mycelial plug. e The mycelial growth of strains on V8 media for 5 days after removal of the two-day cellophane culture. Bars represent the mean ± standard error from at least six seedlings. All statistical analyses were conducted using DPS software 7.05. Differences between the means were determined by the least significant difference (LSD) multiple range test at p = 0.05