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Fig. 2 | Phytopathology Research

Fig. 2

From: The ankyrin repeat-containing protein PlANK1 mediates mycelial growth, oospore development, and virulence in Peronophythora litchii

Fig. 2

CRISPR/Cas9-mediated knockout of PlANK1. a A single guide RNA targeting the PlANK1 coding region was designed to disrupt PlANK1. b and c Three independent deletion mutants were identified by genomic PCR and confirmed by sequencing. d Relative expression levels of PlANK1 were determined by RT-qPCR in WT, CK, KO11, KO14, KO39, and CM38. The constitutive expression of PlActin was used as a reference gene, and expression levels were normalized using the WT values as 1. Data represent means ± SD. The experiment was repeated three times independently. Asterisks denote significant differences between PlANK1 knockout mutants and the WT (** p < 0.01)

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Phytopathology Research

ISSN: 2524-4167