Fig. 6

Inoculation assay of the PlANK1 knockout mutants. a One hundred zoospores of the WT, CK, and PlANK1 knockout mutant and complementation strains were inoculated on tender litchi leaves (n > 30 for each strain) for 48 h at 25 ℃ in the dark. Images are two representatives for each instance. b Lesion length measured after 48 hpi. Different letters represent significant differences (p < 0.01; Duncan’s Multiple Range Test). c One hundred zoospores of the WT, CK, and PlANK1 knockout mutants and complemented strain were inoculated on litchi fruit (n > 30 for each strain) for 72 h at 25 ℃ in the dark. Images are three representatives for each instance. d Lesion length measured after 72 hpi. Different letters represent significant differences (p < 0.01; Duncan's Multiple Range Test). Experiments were repeated three times independently