Fig. 7
PlANK1 regulates the nitrogen assimilation pathway in Peronophythora litchii. a and b Gene ontology and KEGG enrichment analysis of the DEGs. c The WT, KO11, and KO39 strains were grown for 7 d on Plich medium or defined media with NO3–, NO2–, and NH4+, glutamine (Gln) and proline (Pro). d Inhibition rates from the above colonies at 7 d post-inoculation. Data represent means ± SD. The experiment was repeated three times independently, with three technical replications per biological repeat. Asterisks denote significant differences between the PlANK1 knockout mutants and WT strain (**p < 0.01). e The application of Pro and Gln in the infection stage. Zoospore suspension containing 10 mM Pro or Gln was inoculated on litchi leaves (n > 12 for each strain) for 36 h at 25 ℃ in the dark. f Lesion length measured after 36 hpi. Different letters represent significant differences (p < 0.01; Duncan's Multiple Range Test). The experiment was repeated three times independently