Skip to main content
Fig. 2 | Phytopathology Research

Fig. 2

From: Development of novel specific molecular markers for the Sw-5b gene to assist with tomato spotted wilt virus-resistant tomato breeding

Fig. 2

Detection of the Sw-5b gene and genotype determination of tomato varieties using Sw-5b-specific molecular markers. a Detection and genotyping of Sw-5b gene in six tomato varieties using PCR with Sw-5b-specific molecular markers. Genomic DNA from Nicotiana benthamiana was used as the negative control. b Dot-ELISA to detect TSWV infection in tomato plants. Tomato plants were inoculated with TSWV and uninoculated upper leaves were collected and subjected to TSWV infection detection at 14 dpi. c RT-PCR to confirm TSWV infection in tomato plants. Uninoculated upper leaves of TSWV-infected tomato plants were subjected to RT-PCR analysis at 14 dpi. TSWV-infected tomato leaves were used as positive control (+), while leaves from uninfected healthy tomato plants were used as negative control (−)

Back to article page

Phytopathology Research

ISSN: 2524-4167