Fig. 5

Identification of Pss van Hall effectors that inhibit PTI in N. benthamiana. Leaves of five-week-old N. benthamiana were infiltrated with Pst DC3000 D36E harboring the pPSE vector with GFP or different Pss van Hall effectors. Samples were collected 6 h later for RNA extraction and then qPCR using the PTI marker genes a Pti5 and b ACRE31, with ACTIN as an endogenous control. CK, samples without infection. Data are shown as the mean ± SD (n = 3). c ROS production was performed at 15 h post-inoculation using L-012. Data are shown as the mean ± SD (n = 8). d The bacterial population was measured at 4 days post-infiltration. Data are shown as the mean ± SD (n = 8). All experiments were repeated three times with similar results. Significant differences were detected using Student's t test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001