Fig. 2
Scopoletin could inhibit B. cinerea infection. a Symptoms of tomato leaves with B. cinerea (105 conidia/mL) infection following treatment with 50 μg/mL or 100 μg/mL scopoletin. The upper lanes of tomato leaves were unpretreated with scopoletin, while the lower lanes of tomato leaves were pretreated with 50 μg/mL or 100 μg/mL scopoletin for 2 h. The conidia suspension without treatment was used as the control (nontreatment, NT). The diameters of disease lesions on tomato leaves were measured after 3 d of incubation. b Onion epidermis penetration by conidia of B. cinerea treated with 50 μg/mL or 100 μg/mL scopoletin. The photos were taken after 16 h of inoculation. c Scopoletin reduces appressorium formation in B. cinerea. Conidia were harvested and suspended in 10 mM fructose. The photos were taken after 8 h of inoculation at 25℃. The appressoria were indicated with red arrows. d Scopoletin impairs infection cushion development in B. cinerea. Mycelium plugs taken from the edge of the 2-day-old culture of B. cinerea were placed on clean slides and incubated at 25℃ for 36 h. The red curves indicated the edge of mycelium plugs, and the blue curves indicated the sites that are 600 μm away from the edge of mycelium plugs. e Number of infection cushions at the sites 600 μm away from the mycelium plugs. Values on the bars followed by different letters are significantly different at P = 0.05