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Fig. 4 | Phytopathology Research

Fig. 4

From: Scopoletin negatively regulates the HOG pathway and exerts antifungal activity against Botrytis cinerea by interfering with infection structures, cell wall, and cell membrane formation

Fig. 4

Scopoletin affects cell wall and cell membrane formation. a Propidium iodide (PI) fluorescence assay of B. cinerea mycelia. Mycelia were treated with 50 μg/mL scopoletin for 3 h. b PI fluorescence assay of B. cinerea conidia. Conidia were harvested and treated with 50 μg/mL scopoletin for 3 h. c Calcofluor white (CFW) staining of B. cinerea conidia. Conidia were harvested and treated with 50 μg/mL scopoletin for 3 h. d Scopoletin treatment significantly increased the protoplast formation of B. cinerea. Fresh hyphae were first treated with 50 μg/mL scopoletin for 3 h, and then incubated for 2 h in 0.6 M KCl containing 0.25% lysing enzymes before microscopic examination. Values on the bars followed by different letters are significantly different at P = 0.05

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Phytopathology Research

ISSN: 2524-4167