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Fig. 5 | Phytopathology Research

Fig. 5

From: Scopoletin negatively regulates the HOG pathway and exerts antifungal activity against Botrytis cinerea by interfering with infection structures, cell wall, and cell membrane formation

Fig. 5

Scopoletin interferes with the HOG pathway. a Sensitivity of B05.10 to 1 M NaCl, 0.2 μg/mL iprodione, 10 mM H2O2, and 2 μg/mL triadimefon with/without 50 μg/mL scopoletin. The morphology of the strains was photographed and measured after the plates were incubated at 25℃ for 36 h. *: significant at P < 0.05. b Morphology of wild-type strain and BcPtc1 deletion mutant ΔBcPtc1 with/without 50 μg/mL scopoletin. The morphology of the strains was photographed and measured after the plates were incubated at 25℃ for 36 h. Values on the bars followed by different letters are significantly different at P = 0.05. c Scopoletin treatment decreases BcSak1 phosphorylation of B05.10 in response to NaCl and iprodione. Fresh mycelia were cultured in PDB broth for 30 h and treated with 1 M NaCl, 0.2 μg/mL iprodione, and 50 μg/mL scopoletin for another 1 h, as indicated in the figure

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Phytopathology Research

ISSN: 2524-4167