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Fig. 8 | Phytopathology Research

Fig. 8

From: Uncovering the mechanisms underlying pear leaf apoplast protein-mediated resistance against Colletotrichum fructicola through transcriptome and proteome profiling

Fig. 8

Functional evaluation of PbrGlu1 in pear. a Functional validation of PbrGlu1 signal peptide. The strains were cultured on YPDA, CMD-W, or YPRAA medium for two days. Invertase enzymatic activity was assessed by converting 2,3,5-triphenyl tetrazolium chloride (TTC) into insoluble red-colored 1,3,5-triphenyl formazan. b Phenotype of CK and TRV2-PbrGlu1 during C. fructicola infection of pear leaves at 3 dpi. c Disease spot diameter in TRV2-PbrGlu1 leaves and CK leaves. d Expression patterns of CK and TRV2-PbrGlu1 before and after inoculation. e Changes in hydrogen peroxide content. f Changes in SOD activity. * indicated significant differences, * p < 0.05, ** p < 0.01

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Phytopathology Research

ISSN: 2524-4167