Fig. 6

FpOGT influences the alternative splicing of FpGCK-AS1 and thus regulates its expression levels. a A simplified model for mutual exclusivity splicing of FpGCK. The numbers above the graph indicate the location of each exon of FpGCK on the chromosome. b RNA-seq reads mapping to FpGCK-AS1 and FpGCK-AS2 isoform. c The expression level of FpGCK-AS1 in WT or ΔFpOGT detected with RT-PCR (top) and RNA-seq analyses (bottom). d Quantification of FpGCK-AS1-GFP expression in WT and ΔFpOGT strains by western blot analysis (top). Relative band intensity was normalized to FpGAPDH (bottom). Uncropped gels and blots in c and d are shown in Additional file 2: Figure S7. e and d Disease phenotype and disease index of the WT-, ΔFpGCK-, ΔFpGCK-C-inoculated alfalfa seeds. Asterisks indicate a significant difference (t-test, *P < 0.05). g Molecular docking graph of the interaction between FpOGT and FpGCK. Pink, FpOGT model; Green, FpGCK model. Enlarged graphs next to the docking model show the hydrogen bonds interaction with blue bars