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Fig. 8 | Phytopathology Research

Fig. 8

From: FpOGT is required for fungal growth, stress response, and virulence of Fusarium proliferatum by affecting the expression of glucokinase and other glucose metabolism-related genes

Fig. 8

Overexpression of FpGCK partially restores the defects of ΔFpOGT in growth, virulence, and glucose metabolism. a Colony growth and diameters of WT, ΔFpOGT, and ΔFpOGT::FpGCK-GFP grown on PDA at 28 °C for 7 d. b and c Disease phenotypes and disease index of the WT-, ΔFpOGT-, ΔFpOGT::FpGCK-GFP-inoculated alfalfa seeds. d Relative expression of genes encoding key enzymes in glucose metabolism in WT, ΔFpOGT, and ΔFpOGT::FpGCK-GFP strains. Relative expression is normalized to FpActin. e FpGCK-GFP and Mito-Tracker fluorescence signals. White arrows indicate linescan graph analysis areas. Scale bars: 5 μm. f Linescan graphs showing the colocalization of fluorescence signals from FpGCK-GFP and Mito-Tracker. Data shown are means + SD. Asterisks indicate the significant difference compared with WT strain (t-test, *P < 0.05)

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Phytopathology Research

ISSN: 2524-4167