Fig. 4
LtGPX3 is required for resistance to oxidative stress. a Mycelial morphology of WT, two deletion mutants, and the complementation strain ΔLtgpx3/GPX3, cultured on PDA media supplemented with 5 mM H2O2, 0.68 mM cumene H2O2, 0.5 mM TBHP (tert-butyl-hydroperoxide), 5 mM SNP (sodium nitroferricyanide dihydrate), or water (mock) for 24 h. b Inhibition rate of fungal growth on PDA with oxidants compared to the mock. Letters on top of bars represent statistically significant differences at P < 0.05. Data are means ± SD of three biological replicates. c and d Time-course response of LtGPX3 transcripts to H2O2 or TBHP exposure. Transcript levels were normalized with reference gene LtTUB and are displayed relative to the transcript level in samples at time 0 (which was therefore set to 1). Asterisks indicate a significant difference from the initial point (0 min) at P < 0.01. Data are means ± SD of three biological replicates