Fig. 1

Specificity, broad-spectrum, and sensitivity analyses of the prepared two MAbs by indirect-ELISA.a Specificity and broad-spectrum analyses of the prepared two MAbs by indirect-ELISA using ten Xoc strains (Xoc RS105, Xoc BLS256, Xoc oxy01, Xoc oxy02, Xoc oxy04, Xoc oxy05, Xoc JS, Xoc GX, Xoc AH, and Xoc YN) and ten negative control bacterial strains (X. oryzae pv. oryzae PXO86, X. oryza pv. oryzae Y2, X. citri pv. citri 29–1, X.campestris CGMCC 1.3408, X. sacchari ACCC 10416, X.axonopodis CCTCC AB 2018263, X. albilineans FJ1, Burkholderia glumae Os48, Pantoea ananatis F163 and Acidovorax oryzae CGMCC 1.1728) as the test samples. The optical density at 405 nm (OD₄₀₅) value of each treatment was measured at 30 min after the addition of the substrate solution and is presented as the mean values ± standard deviation (SD) from three independent assays. Two asterisks (**) indicate a significant difference (P < 0.01; Student's t-test). b Sensitivity analyses of the prepared two MAbs by indirect-ELISA using serially two-fold diluted Xoc strain RS105 and Xoo strain PXO86 suspensions (from 1 × 10⁷ to 76.3 CFU/mL) as the test samples. The OD₄₀₅ value of each treatment is presented as the mean values ± SD from three independent assays