Fig. 2

Specificity, broad-spectrum, and sensitivity analyses of the developed two Dot-ELISAs using MAbs 11D2 and 11B6 as the detection antibodies. a Specificity and broad-spectrum analyses of the two developed Dot-ELISAs for the detection of Xoc. A1–10 represent Xoc RS105, Xoc BLS256, Xoc oxy01, Xoc oxy02, Xoc oxy04 Xoc oxy05, Xoc JS, Xoc GX, Xoc AH, and Xoc YN, used as the positive bacterial strains. B1–10 represent X. oryzae pv. oryzae PXO86, X. oryza pv. oryzae Y2, X. citri pv. citri 29–1, X.campestris CGMCC 1.3408, X. sacchari ACCC 10416, X.axonopodis CCTCC AB 2018263, X. albilineans FJ1, Burkholderia glumae Os48, Pantoea ananatis F163, and Acidovorax oryzae CGMCC 1.1728, used as the negative control bacterial strains. Each diluted suspension (2 µL) was separately spotted on the nitrocellulose membranes and then tested by the two developed Dot-ELISAs. Purple dots indicate positive reactions, while no color dots indicate negative reactions. b Sensitivity analyses of the two developed dot-ELISAs for the detection of Xoc. Xoc strain RS105 and Xoo strain PXO86 (as negative control) suspensions were serially two-fold diluted, and each resulting diluted suspension (2µL) was loaded onto a nitrocellulose membrane for the Dot-ELISA test. c Sensitivity analyses of the two developed dot-ELISAs for the detection of Xoc in rice plants. A homogenate from a Xoc-infected or an uninfected rice leaf tissue was serially two-fold diluted, and each dilution (2µL) was loaded onto a nitrocellulose membrane for Dot-ELISA test. d Sensitivity analysis of conventional PCR for Xoc detection. Serially diluted Xoc strain RS105 suspensions were tested by PCR using Xoc-forward primer/Xoc-reverse primer to amplify a specific 694 bp gene fragment. e Sensitivity analysis of PCR for a Xoc-infected rice leaf tissue, same as in Fig. 2c