Fig. 4

Specificity, broad-spectrum, and sensitivity analyses of the developed CGICS for Xoc detection. a Specificity and broad-spectrum of the developed CGICS were determined using ten different Xoc strains and ten different negative control bacterial strains. 1–10 represent Xoc RS105, Xoc BLS256, Xoc oxy01, Xoc oxy02, Xoc oxy04 Xoc oxy05, Xoc JS, Xoc GX, Xoc AH, and Xoc YN, respective. 11–20 represent X. oryzae pv. oryzae PXO86, X. oryza pv. oryzae Y2, X. citri pv. citri 29–1, X.campestris CGMCC 1.3408, X. sacchari ACCC 10416, X.axonopodis CCTCC AB 2018263, X. albilineans FJ1, Burkholderia glumae Os48, Pantoea ananatis F163, and Acidovorax oryzae CGMCC 1.1728 respectively. Each bacterial suspension (100 µL) was loaded into the sample pads of the CGICSs, and then the color of the C and T lines was observed with the naked eye in 5–10 min. b The sensitivity of the developed CGICS was determined using serially two-fold diluted bacterial suspensions of Xoc strain RS105. c Sensitivity analysis of the developed CGICS for the detection of Xoc in a rice leaf tissue. A homogenate from a Xoc-infected was serially two-fold diluted, and each dilution (100 µL) was loaded into the sample pads for the CGICS test. A homogenate from an uninfected rice leaf tissue was used as the negative control