Fig. 1

UvHOS2 is required for hyphal growth, conidial development and germination in U. virens. a Colony morphology of the wild-type, ΔUvhos2 mutant, and CΔUvhos2 complemented strains after culturing on PSA plates for 14 d. b The interseptal distance in the hyphae of the wild-type, ΔUvhos2-51, ΔUvhos2-52, and CΔUvhos2 strains was observed by fluorescence microscopy. The hyphae were stained with Calcofluor White after conidia were germinated for 2 d. Scale bars: 20 μM. c The average interseptal distance of the wild-type, ΔUvhos2-51, ΔUvhos2-52, and CΔUvhos2 strains. A total of 100 interseptum compartments were randomly selected and measured. Data are presented as mean ± standard error (SEM) (n = 100). d The number of conidia generated by the wild-type, ΔUvhos2-51, ΔUvhos2-52, and CΔUvhos2 strains after culturing in PSB medium for 7 d. Data are shown as mean ± SEM (n = 3). e Spore germination was observed with microscopy for the wild-type, ΔUvhos2-51, ΔUvhos2-52, and CΔUvhos2 strains from 3 to 12 h. Scale bars: 5 μM. f The conidial germination rates of the wild-type, ΔUvhos2-51, ΔUvhos2-52, and CΔUvhos2 strains at the indicated time points. A total of 100 spores were randomly selected and observed. Data are shown as mean ± SEM (n = 3). Different lowercase letters indicate statistically significant differences (P < 0.05, one-way ANOVA with Duncan’s multiple comparisons)