Fig. 4

UvHOS2 is involved in histone deacetylation at H3K9, H3K14, H3K27, and H3K56 in U. virens. a Western blot analyses to detect the acetylation levels at different Lys residues of H3 and H4 in the wild-type, mutant, and complemented strains using the indicated site-specific anti-acetylation antibodies. β-actin, H3, and H4 proteins were also detected as internal controls through western blotting. b The relative acetylation levels at different Lys residues in H3 and H4 were quantified by densitometry using ImageJ. Data from three biological replicates are shown as mean ± SEM (n = 3). Different lowercase letters indicate statistically significant differences in the site-specific acetylation levels among different strains (P < 0.05, one-way ANOVA with Duncan’s multiple comparisons)