Fig. 6
UvHOS2 regulates secondary metabolism and ustilaginoidin biosynthesis in U. virens. Heatmaps to show the expression levels of the genes involved in the biosynthesis of ustilaginoidins a and sorbicillinoids b, and the genes encoding cytochrome P450 c and non-ribosomal peptide synthetases d associated with secondary metabolism in the wild-type and ΔUvhos2 mutant strains. Transcriptome analyses were performed with three replicates. The color column indicates the levels of gene expression that were scaled using the "Normalized" method, with red representing higher expression levels and blue indicating lower expression levels. e, f RT-qPCR assays to validate the expression levels of some cytochrome P450 genes, PKS genes and redox-related genes, and ustilaginoidin biosynthesis-related genes in the wild-type, ΔUvhos2, and complemented strains. The significance of differences was analyzed using one-way ANOVA with Duncan’s multiple comparisons. Different lowercase letters indicate statistically significant differences (P < 0.05)