Fig. 2

Gmmrlk32 mutant display enhanced PTI and disease resistance. a The Gmmrlk32 and Gmlmm1 mutants showed enhanced flg22-induced ROS. Leaves of the indicated plants at 2 weeks old were subjected to flg22 -induced ROS examination (Mean ± SD, n ≥ 6, Student’s t-test; ** p < 0.01, *** p < 0.001). b The Gmmrlk32 and Gmlmm1 mutants showed enhanced flg22-induced MAPK activation. Leaves of the indicated plants at 2 weeks old were subjected to flg22-induced MAPK examination by anti-pERK immunoblots. Quantitative analysis of MAPK intensity was performed using imageJ and numbers on top of the gel blots indicate ratio of MAPK/Rubisco. c The Gmmrlk32 and Gmlmm1 mutants exhibited enhanced resistance to P. syringae pv. glycinea (Psg) infection. Leaves of 2-week-old soybean plants were infected with Psg, and the bacteria number was calculated at 0 and 3 dpi (Mean ± SD, n ≥ 6, Student’s t-test; * p < 0.05, ***p < 0.001). d The Gmmrlk32 and Gmlmm1 mutants exhibited enhanced resistance to P. sojae infection. Leaves of 2-week-old soybean plants were infected with P. sojae strain P7076, and the lesions were examined 2 days later via trypan blue staining. Scale bars: 1 cm. The experiment repeated three times