Fig. 3

GmMRLK32 interacts with GmFLS2 and GmBAK1. a GmMRLK32 interacts with GmFLS2 and NbFLS2 in N. benthamiana by luciferase complementation image (LCI) assays. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay, and LCI assays were performed to examine the protein interactions. GmILPA1 was used as a negative control. The protein expression levels are shown in Additional file 5: Figure S5a. b GmMRLK32 interacts with GmFLS2 and NbFLS2 in N. benthamiana, as determined via co-IP assays. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay, and protein interactions were examined by co-IP assay. c GmMRLK32 interacts with GmBAK1 and NbBAK1 in N. benthamiana by LCI assay. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay, and LCI assays were performed to examine the protein interactions. The protein expression levels are shown in Additional file 5: Figure S5b. d GmMRLK32 interacts with GmBAK1 and NbBAK1 in N. benthamiana by co-IP assays. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay, and protein interactions were examined by co-IP assay. e The GmMLRK32-GmFLS2 interaction was dynamically regulated by flg22. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay, treated with 1 μM flg22, and protein interactions were examined by co-IP assay. f The GmMRLK32-GmBAK1a interaction was not affected by flg22 treatment. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay, treated with 1 μM flg22, and protein interactions were examined by co-IP assay. The experiments were performed three times with similar results