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Fig. 4 | Phytopathology Research

Fig. 4

From: A pair of soybean malectin-like domain-containing receptor-like kinases jointly regulate pattern-triggered immunity by forming hetero-oligomers

Fig. 4

GmMRLK32 suppresses flg22-iduced GmFLS2-GmBAK1 interactions. a GmMRLK32 and GmLMM1 suppress flg22-induced GmFLS2-GmBAK1a interaction. GmFLS2 and GmBAK1a were co-expressed in N. benthamiana along with GmLMM1 or GmMRLK32, treated with or without flg22, and the GmFLS2-GmBAK1 interactions were examined by co-IP assays. b GmMRLK32 suppresses flg22-induced GmFLS2-GmBAK1b interaction. GmFLS2 and GmBAK1b were co-expressed in N. benthamiana along with GmMRLK32, treated with or without flg22, and subjected to co-IP assays. c GmMRLK32 inhibits GmFLS2-GmBAK1a interaction by LCI assays. The indicated constructs were transiently expressed in N. benthamiana and LCI assays were performed to examine the protein interactions. The protein expression levels were shown by immunoblotting. (Mean ± SD, n ≥ 8, Student’s t-test; ** p < 0.01, *** p < 0.001). d GmMRLK32 suppresses flg22-induced GmFLS2-GmBAK1a interaction in a kinase-independent manner. GmFLS2 and GmBAK1a were co-expressed in N. benthamiana along with GmMRLK32 or GmMRLK32K562E, treated with or without flg22, and the GmFLS2-GmBAK1a interactions were examined by co-IP assays

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Phytopathology Research

ISSN: 2524-4167