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Fig. 5 | Phytopathology Research

Fig. 5

From: A pair of soybean malectin-like domain-containing receptor-like kinases jointly regulate pattern-triggered immunity by forming hetero-oligomers

Fig. 5

GmMRLK32 specifically interacts with GmLMM1. a GmMRLK32 interacts with GmLMM1 in N. benthamiana by LCI assay. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay, and LCI assays were performed to examine the protein interactions. The protein expression levels were examined by immunoblotting as shown in Additional file 5: Figure S5c. b GmMRLK32 dynamically interacts with GmLMM1 in N. benthamiana by co-IP assay. GmMRLK32-HA and GmLMM1-FLAG were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay, treated with or without flg22, and protein interactions were examined by co-IP assay. c flg22 specifically regulates GmMRLK32-GmLMM1 interaction. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay, treated with or without flg22, and protein interactions were examined by co-IP assay

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Phytopathology Research

ISSN: 2524-4167