Fig. 6

GmMRLK32 and GmLMM1 jointly regulate PTI and disease resistance. a and b Co-expression of GmMRLK32 and GmLMM1 showed stronger suppression effect on PTI than expression of GmMRLK32 or GmLMM1 alone. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assays and subjected to flg22-induced ROS and MAPK examination. (Mean ± SD, n ≥ 8, Student’s t-test; different letters indicate significant difference at p < 0.01). Quantitative analysis of MAPK intensity was performed by imageJ and numbers on top of the gel blots indicate ratio of MAPK/Rubisco. c N. benthamiana plants co-expressing GmMRLK32 and GmLMM1 were more susceptible than plants expressing GmMRLK32 or GmLMM1 alone. The indicated constructs were expressed in N. benthamiana by Agrobacterium-mediated transient expression assay for one  day; The resulted tissue was inoculated with P. capsici strain LT263, and photographed under UV light 48 h later. The lesion area was measured. (Mean ± SD, n ≥ 8, Student’s t-test; different letters indicate significant difference at p < 0.05). d Co-expression of GmMRLK32 and GmLMM1 showed stronger suppression effect on XEG1-induced cell death, compared to expression of GmMRLK32 or GmLMM1 alone. The indicated constructs were transiently expressed in N. benthamiana 1 day before infiltration of XEG1, and cell death phenotypes were examined two days later. e A model illustrating that GmMRLK32 and GmLMM1 regulating GmFLS2-GmBAK1 interaction by forming hetero-oligomers