Fig. 2
RcCDI1 and BcIEB1 induced resistance to P. capsici and P. parasitica in N. benthamiana plants. a Purification of recombinant His-tagged RcCDI1 and BcIEB1 proteins from E. coli. The RcCDI1-His and BcIEB1-His proteins were marked with asterisk. b and c RcCDI1 and BcIEB1 induced plant resistance to P. capsici strain LT263 and P. parasitica strain Pp025 in N. benthamiana. d and e RcCDI1 and BcIEB1 induced plant resistance to fungal pathogen S. sclerotiorum and B. cinerea in N. benthamiana. Leaves of N. benthamiana were infiltrated with BcIEB1-His, RcCDI1-His, or GFP-His for 1 d. The leaves were inoculated with indicated pathogens and photographed 36–48 h later. Lesion areas were measured and calculated (mean ± SD, n ≥ 6; Student’s t-test; *, P < 0.05, **, P < 0.01, ***, P < 0.001). Scale bars: 1 cm. All experiments were performed three times with similar results