Fig. 3

RcCDI1- and BcIEB1-induced plant immune responses required NbBAK1 and NbSOBIR1. a and b RcCDI1 and BcIEB1 induced the expression of plant defense genes NbCYP71D20 and NbPTI5. The 5-week old N. benthamiana plants were infiltrated with RcCDI1 and BcIEB1 at the concentration of 1 μM. c RcCDI1 induced cell death in N. benthamiana in a NbBAK1- and NbSOBIR1-dependent manner. The indicated constructs were transiently expressed in leaves of Nbsobir1 and NbBAK1-silencing plants. The cell death phenotype was visualized 4 d after. INF1 was used as a positive control. d BcIEB1-induced expression of NbPTI5 required NbSOBIR1. BcIEB1 (1 μM) was infiltrated into the leaves of 5-week old WT and Nbsobir1 mutant plants, and leaf tissues collected at 0 h and 6 h were used for the assay. e BcIEB1-induced expression of NbPTI5 required NbBAK1. BcIEB1 (1 μM) was infiltrated into the 5-week old TRV: GFP and NbBAK1-silencing N. benthamiana plants, and leaf tissues collected at 0 h and 6 h were used for the assay. For gene expression analysis, total RNA was extracted and subjected to qRT-PCR assay, and fold changes before and after elicitor treatment were calculated (mean ± SD, Student’s t-test; ***, P < 0.001). All experiments were performed three times with similar results