Fig. 5

Combined application of BcIEB1 with pyraclostrobin or azoxystrobin significantly alleviated plant disease symptoms caused by P. capsica. a and b Leaves of N. benthamiana were infiltrated with BcIEB1 (1 µM), different concentrations of pyraclostrobin, or BcIEB1(1 µM) plus pyraclostrobin (5 µg/mL) for 1 d. The leaves were inoculated by P. capsici strain LT263 and photographed 36–48 h later under UV light. The lesion areas were measured and calculated (mean ± SD, n ≥ 6, different letters indicate significant difference at P < 0.05, one-way ANOVA followed by Tukey's post hoc test). Scale bars: 1 cm. c and d Joint application of BcIEB1 with azoxystrobin significantly reduced plant susceptibility to P. capsici. The assays were performed as in a (mean ± SD, n ≥ 6, different letters indicate significant difference at P < 0.05, one-way ANOVA followed by Tukey's post hoc test). Scale bars: 1 cm. All experiments were performed three times with similar results