Fig. 7

Combined application of BcIEB1 and pyraclostrobin alleviated plant disease symptoms caused by P. capsici and P. infestans and allows lower usage of fungicide in tomato and potato plants. a Combined application of BcIEB1 and pyraclostrobin reduced potato susceptibility to P. infestans. Leaves of potato were infiltrated with BcIEB1 (1 μM), pyraclostrobin (10 μg/mL), or BcIEB1(1 μM) and pyraclostrobin (5 μg/mL) for 1 d. The leaves were inoculated by P. infestans strain pd21355-Hap1 and photographed under UV light 7 d later. The lesion areas were measured and calculated (mean ± SD, n ≥ 6, different letters indicate significant difference at P < 0.05, one-way ANOVA followed by Tukey's post hoc test). Scale bars: 1 cm. b Combined application of BcIEB1 and pyraclostrobin reduced tomato susceptibility to P. capsici. Leaves of tomato were infiltrated with BcIEB1 (1 μM), Pyraclostrobin (10 μg/mL), or BcIEB1(1 μM) plus Pyraclostrobin (5 μg/mL) for 1 d. The leaves were inoculated by P. capsici strain LT263 and photographed under UV light 2 d later. The lesion areas were measured and calculated (mean ± SD, n ≥ 6, different letters indicate significant difference at P < 0.05, one-way ANOVA followed by Tukey's post hoc test). Scale bars: 1 cm. All experiments were performed two times with similar results